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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory encounters a Gram-negative bacillus isolated from a bloodstream infection. The isolate exhibits resistance to multiple antibiotics, including carbapenems. Further phenotypic testing reveals the production of a metallo-beta-lactamase (MBL). Which of the following antibiotic combinations might still retain activity against this isolate?

A) Aztreonam-avibactam
B) Aztreonam
C) Ceftazidime-avibactam
D) Piperacillin-tazobactam

2. A traveler returning from sub-Saharan Africa presents with cyclical fevers, chills, and sweats. A Giemsa- stained peripheral blood smear reveals multiple ring forms within red blood cells, some applique forms, and occasional banana-shaped gametocytes. No Schüffner's dots or red blood cell enlargement is observed. Which Plasmodium species is the most likely cause?

A) Plasmodium falciparum
B) Plasmodium vivax
C) Plasmodium malariae
D) Plasmodium ovale

3. A patient develops a severe skin infection after a puncture wound sustained while gardening. Culture of the wound exudate grows a Gram-positive, spore-forming bacillus that is anaerobic and produces a potent neurotoxin causing sustained muscle contraction. The MOST likely organism is:

A) Bacillus anthracis
B) Clostridium perfringens
C) Clostridium botulinum
D) Clostridium tetani

4. A clinical microbiology laboratory is performing Gram staining on a sputum sample. After adding the crystal violet stain, a delay in the washing step with decolorizer (alcohol-acetone) would MOST likely result in:

A) Increased background staining.
B) Poor adherence of the stain to Gram-positive bacteria.
C) Gram-positive bacteria appearing pink.
D) Gram-negative bacteria appearing purple.

5. Under the Clinical Laboratory Improvement Amendments (CLIA), participation in an approved external Proficiency Testing (PT) program serves primarily to:

A) Fulfill requirements for laboratory accreditation by The Joint Commission only.
B) Provide an objective, ongoing assessment of the accuracy and reliability of the laboratory's testing compared to its peers.
C) Determine the reference intervals (normal ranges) for quantitative assays performed by the laboratory.
D) Validate the performance characteristics (e.g., sensitivity, specificity) of newly implemented test methods.

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